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02 octobre 2012

Most two-hybrid system often simultaneously use two or even three reported gene

    Developed a variety of two-hybrid system is mostly based system established by Fields et al. These new systems are mainly on the reporter gene, "bait" expression vector "prey" expression vector made some improvements. One of the important improvement is the introduction of additional reporter genes HIS3 gene, such as the widely adopted. Transformed with the yeast cells of the HIS3 reporter gene HIS3 be started, only when the expression can grow on selective medium lacking histidine. HIS3 transcriptional expression of the reporter gene is activated by the "bait" and "prey" interaction. Most two-hybrid system often simultaneously use two or even three reporter genes, one of which is LacZ. After transformation the gene same activator of transcription factor binding sites in the promoter region and can therefore be the same transcription activating factor (as described above, the Gal4 protein) activation. By double or multiple choice both to improve the detection sensitivity and reduce false positives. Other improvements made for "bait" or "prey" expression vector.

Posté par Hense007 à 17:52 - Commentaires [0] - Permalien [#]


23 septembre 2012

Knowledge of drug contraindications

(1) of proprietary Chinese medicines Yixin Dan, Shexiangbaoxin pill, Liu Wei Di Huang Wan is not appropriate and western medicine propafenone, quinidine same clothes, can lead to cardiac arrest.

(2) Chinese medicine tiger bone wine, ginseng wine, Shujin vibrant wine with western medicine luminal sedative painkiller same clothes enhance the inhibitory effect on the central nervous system and dangerous.

(3) Danshen tablets should not be used in combination with Weishuping Danshen tablets, the main ingredient is tanshinone, Salvia phenol to form aluminum conjugates can not be easily absorbed in the intestine, reducing the efficacy of aluminum hydroxide contained with Weishuping.

(4) kelp tablets should not be combined with isoniazid iodine kelp film, oxidation reaction occurs with isoniazid under acid conditions, the formation of isonicotinic acid halide and nitrogen, loss of the function of the anti-TB.

(5) active Dan Hong contiguous, Fritillaria Loquat syrup should not be used in combination with atropine, caffeine, aminophylline. Aconitum, Coptis, Fritillaria alkaloid-containing component due to the former, and the latter with the service, it is likely to increase the toxicity of a drug poisoning.

Posté par Hense007 à 12:10 - Commentaires [0] - Permalien [#]

14 septembre 2012

Epidermal growth factor and fibroblast growth factor combined with the repair of diabetic wound healing process

    Objective: joint application of recombinant human epidermal growth factor and basic fibroblast growth factor, to explore the impact of diabetic wound healing process.
 
:    Select 2001-05/2005-03 Guangxi Medical University, Burns and Plastic Surgery Rehabilitation Center treated 29 patients with diabetic foot ulcers in patients voluntarily signed informed consent. Random table, 29 patients were randomly divided into four groups: seven cases of the combined treatment group, eight cases of recombinant human epidermal growth factor group, basic fibroblast growth factor group of 6 patients, saline control group of eight cases. Groups of patients in the treatment record before the fasting blood glucose control 11.1mmol / L, with infection after effective antibiotics intravenously to infection control, wound curettage pathologic granulation. Combination group were given topical recombinant human epidermal growth factor 1500IU / Spraying the basic fibroblast growth factor 720AU /; recombinant human epidermal growth factor group treated with topical recombinant human epidermal growth factor 1500IU /; basic fibroblast The growth factor group was given outside the spray 720AU / basic fibroblast growth factor; saline control group only saline wipe the wound. Each group dressing the next day dressing, 3, 7 and 14 days after treatment wound epithelial Portuguese line and the maturity of the granulation tissue of wound.
 
     Results: The selection of the 29 cases of patients with diabetic foot, all the analysis of results. ① different times after wound treatment groups wound healing rate comparison: three days after wound treatment groups basically similar (P) 0.05); wound treatment seven days compared with the saline control group, the combined treatment group and recombinant human epidermal growth factor group significantly increased (P <0.05>; 14 days after the treatment of the wound with saline control group, the combined treatment group, recombinant human epidermal growth factor group, basic fibroblast growth factor group were significantly improved (21.67 ± 1.53)%, (33.50 ± 1.56)%, (28.77 ± 1.50)%, (23.73 ± 1.20)% (P <0.01 or 0.05 liter to the combination therapy group high most obvious ② wound treatment after different times granulation tissue growth and general observation of the situation: each group 3,7 days growth of granulation tissue in wound management are basically similar to (P) 0.05). wound management 14 days, the combined treatment group wound granulation tissue growth was significantly superior to recombinant human epidermal growth factor group, basic fibroblast growth factor group, saline control group (P <0.05>.

Posté par Hense007 à 14:50 - Commentaires [1] - Permalien [#]

Epidermal growth factor and fibroblast growth factor combined with the repair of diabetic wound healing process

Objective: joint application of recombinant human epidermal growth factor and basic fibroblast growth factor, to explore the impact of diabetic wound healing process.
 
:    Select 2001-05/2005-03 Guangxi Medical University, Burns and Plastic Surgery Rehabilitation Center treated 29 patients with diabetic foot ulcers in patients voluntarily signed informed consent. Random table, 29 patients were randomly divided into four groups: seven cases of the combined treatment group, eight cases of recombinant human epidermal growth factor group, basic fibroblast growth factor group of 6 patients, saline control group of eight cases. Groups of patients in the treatment record before the fasting blood glucose control 11.1mmol / L, with infection after effective antibiotics intravenously to infection control, wound curettage pathologic granulation. Combination group were given topical recombinant human epidermal growth factor 1500IU / Spraying the basic fibroblast growth factor 720AU /; recombinant human epidermal growth factor group treated with topical recombinant human epidermal growth factor 1500IU /; basic fibroblast The growth factor group was given outside the spray 720AU / basic fibroblast growth factor; saline control group only saline wipe the wound. Each group dressing the next day dressing, 3, 7 and 14 days after treatment wound epithelial Portuguese line and the maturity of the granulation tissue of wound.
 
       Results: The selection of the 29 cases of patients with diabetic foot, all the analysis of results. ① different times after wound treatment groups wound healing rate comparison: three days after wound treatment groups basically similar (P) 0.05); wound treatment seven days compared with the saline control group, the combined treatment group and recombinant human epidermal growth factor group significantly increased (P <0.05>; 14 days after the treatment of the wound with saline control group, the combined treatment group, recombinant human epidermal growth factor group, basic fibroblast growth factor group were significantly improved (21.67 ± 1.53)%, (33.50 ± 1.56)%, (28.77 ± 1.50)%, (23.73 ± 1.20)% (P <0.01 or 0.05 liter to the combination therapy group high most obvious ② wound treatment after different times granulation tissue growth and general observation of the situation: each group 3,7 days growth of granulation tissue in wound management are basically similar to (P) 0.05). wound management 14 days, the combined treatment group wound granulation tissue growth was significantly superior to recombinant human epidermal growth factor group, basic fibroblast growth factor group, saline control group (P <0.05>.

Posté par Hense007 à 14:48 - Commentaires [0] - Permalien [#]

     Objective: joint application of recombinant human epidermal growth factor and basic fibroblast growth factor, to explore the impact of diabetic wound healing process.
 
:    Select 2001-05/2005-03 Guangxi Medical University, Burns and Plastic Surgery Rehabilitation Center treated 29 patients with diabetic foot ulcers in patients voluntarily signed informed consent. Random table, 29 patients were randomly divided into four groups: seven cases of the combined treatment group, eight cases of recombinant human epidermal growth factor group, basic fibroblast growth factor group of 6 patients, saline control group of eight cases. Groups of patients in the treatment record before the fasting blood glucose control 11.1mmol / L, with infection after effective antibiotics intravenously to infection control, wound curettage pathologic granulation. Combination group were given topical recombinant human epidermal growth factor 1500IU / Spraying the basic fibroblast growth factor 720AU /; recombinant human epidermal growth factor group treated with topical recombinant human epidermal growth factor 1500IU /; basic fibroblast The growth factor group was given outside the spray 720AU / basic fibroblast growth factor; saline control group only saline wipe the wound. Each group dressing the next day dressing, 3, 7 and 14 days after treatment wound epithelial Portuguese line and the maturity of the granulation tissue of wound.
 
     Results: The selection of the 29 cases of patients with diabetic foot, all the analysis of results. ① different times after wound treatment groups wound healing rate comparison: three days after wound treatment groups basically similar (P) 0.05); wound treatment seven days compared with the saline control group, the combined treatment group and recombinant human epidermal growth factor group significantly increased (P <0.05>; 14 days after the treatment of the wound with saline control group, the combined treatment group, recombinant human epidermal growth factor group, basic fibroblast growth factor group were significantly improved (21.67 ± 1.53)%, (33.50 ± 1.56)%, (28.77 ± 1.50)%, (23.73 ± 1.20)% (P <0.01 or 0.05 liter to the combination therapy group high most obvious ② wound treatment after different times granulation tissue growth and general observation of the situation: each group 3,7 days growth of granulation tissue in wound management are basically similar to (P) 0.05). wound management 14 days, the combined treatment group wound granulation tissue growth was significantly superior to recombinant human epidermal growth factor group, basic fibroblast growth factor group, saline control group (P <0.05>.

Posté par Hense007 à 14:45 - Commentaires [0] - Permalien [#]


06 septembre 2012

Epidermal growth factor in combination with the cell surface receptor

      Epidermal growth factor (Epidermal growth factor, EGF) is the earliest discovered growth factor plays an important role in regulating cell growth, proliferation and differentiation. In humans, the epidermal growth factor protein of 53 amino acid residues, 6 one thousand three disulfide bonds, quality ear Dayton. 

     Epidermal growth factor in combination with the cell surface receptor, the epidermal growth factor receptor (EGFR) functions. [3] combined with the high affinity of the epidermal growth factor receptor to stimulate receptor intrinsic tyrosine kinase activity, which initiates a signal transduction cascade and lead to a variety of biochemical changes in intracellular calcium levels rise, increasing the sugar leaven Solutions and protein synthesis, increase in the expression of certain genes (including epidermal growth factor receptor), eventually leading to DNA synthesis and cell proliferation. 

Posté par Hense007 à 14:39 - Commentaires [0] - Permalien [#]

26 août 2012

EGF of the distribution and expression

      EGF of angiogenesis is thought to play a key role, in part because the receptors and raised VEGFmRNA expression and regulation angiogenesis. Studies have pointed out that EGF and EGF-R continued combination, it can guide EGF-R into degradation. We study found that in normal tissue and high oxygen lung damage vascular endothelial cells are visible EGFR strong positive expression, blood vessels in the wall of the cable shape distribution, identical with those reported in literature. Our experimental results and by many of the reports in the literature that might be involved in EGF after birth lung angiogenesis and promote the alveolar formation

Posté par Hense007 à 16:35 - Commentaires [0] - Permalien [#]

22 août 2012

B-EGFmRNA and protein expression

      B-EGFmRNA and protein expression,in pregnancy d1,the expression of the HB-EGF is higher,the main distribution in endometrial cavity epithelial and glandular epithelium,pregnancy d2~3,the expression of the HB-EGF is very small,at the blastocyst before and after implantation,the expression of the HB-EGF is higher,and a regional distribution,mainly distributed in the lining of the uterus is film side cavity epithelial inside,and in between two blastocysts endometrial cavity epithelial expressed in the quantity is few,HB-EGF may be in mice blastocysts implantation in the process to play important regulatory role. In the pig endometrial cavity epithelium in a HB-EGF expression. In the womb macaques,HB-EGF mainly in endometrial cavity epithelial lining,glandular epithelium and expressed in the muscular layer,middle and embryo implantation in secretion before and after high quantity expression; In one endometrial proliferation in the expression of HB-EGF volume is low,and in secretory endometrium HB-EGF within the expression of high volume,and in the bed before (menstrual cycle d19 ~ 21) to peak,since then,the expression of the HB-EGF quantity and gradually reduce; In the proliferation of the period,HB-EGF mainly in endometrial expressed in the matrix,middle secretion in endometrial cavity epithelial cells top also have HB-EGF expression,HB-EGF in macaques and people may be blastocysts implantation also play an important role in the process

Posté par Hense007 à 17:22 - Commentaires [0] - Permalien [#]

20 août 2012

Heparin-binding nature of EGF-like growth factor ( HB -EGF ) Profile

      HB-EGF is a member of the EGF family of recently discovered one of the first growth factor with heparin affinity chromatography to separate from the macrophage-like U-937 cell-conditioned medium composed of 208 amino acid residues molecular weight of 20 ~ 22kD.HB-EGF gene is located on chromosome 5 and mouse chromosome No.18.HB-EGF expression in many organizations,including lung,heart,kidney,testis and skin.HB-EGF with cell surface heparin sulfate proteoglycan ( HSPG) and EGFR can stimulate cell proliferation and migration.In cell cultures,HB-EGF can promote a number of cells in mitosis,such as mice of BALB - 3T3 into fiber cells,rat liver cells,rabbit kidney epithelial cells,human keratinocyte cells and so on.

Posté par Hense007 à 17:20 - Commentaires [0] - Permalien [#]

30 juillet 2012

Epidermal growth factor,

      Epidermal growth factor is the body of an active substance, consisting of 53 amino living cells, by stimulating epidermal growth factor receptor tyrosine phosphorylation, the skin surface cells to repair hyperplasia, is said to injuries,the loss of the epidermis has an excellent efficacy. Its greatest feature is able to promote cell proliferation and differentiation of new cells to replace the aging and dying cells. EGF can stop bleeding and accelerate the skin and mucosal wound healing, anti-inflammatory analgesic to prevent the effectiveness of the ulcer the stability of the EGF excellent, not easily separated flow at room temperature, and good coordination with the human body of various enzyme effect. The first EGF is mainly used in the medical field, mainly used to promote repair and regeneration of the damaged epidermis, such as the treatment of burns, burns and promote wound healing, repair of the gastrointestinal tract, liver and cornea injury, the efficacy is very significant.

      Growth factors is extremely complex, non-human to take the source of exogenous growth factor, into the impact study of the human body is not too much, the cumulative information is not enough scientific quantifiable representation, required longer years to mature, make sure

      EGF was discovered in 1962 by Dr. Stanley Cohen won a Nobel Prize in 1986.

Posté par Hense007 à 10:36 - Commentaires [0] - Permalien [#]