02 octobre 2012

Most two-hybrid system often simultaneously use two or even three reported gene

    Developed a variety of two-hybrid system is mostly based system established by Fields et al. These new systems are mainly on the reporter gene, "bait" expression vector "prey" expression vector made some improvements. One of the important improvement is the introduction of additional reporter genes HIS3 gene, such as the widely adopted. Transformed with the yeast cells of the HIS3 reporter gene HIS3 be started, only when the expression can grow on selective medium lacking histidine. HIS3 transcriptional expression of the... [Lire la suite]
Posté par Hense007 à 17:52 - Commentaires [0] - Permalien [#]